《植物生理学报》 2017, 53(4): 619-624

武夷岩茶大红袍的组织培养与快速繁殖

田奥磊¹, 高俊杰², 李丹丹¹, 刘建福^1,*, 王明元¹, 徐茂兴³, 张斌⁴

¹华侨大学园艺系, 福建厦门361021; ²泉州市农业局种植业管理站, 福建泉州362000; ³武夷山市茶业局, 福建南平354300; ⁴武夷山市仙茗岩茶厂, 福建南平354300

通信作者：刘建福；E-mail: jianfu@hqu.edu.cn

摘　要：

以武夷岩茶大红袍的无菌种胚为外植体, 通过对其胚的诱导、继代增殖及生根诱导培养的筛选, 建立了完整的组织培养的体系。结果表明: 武夷岩茶大红袍生长最简单的诱导培养基为MS+蔗糖30 g·L^-1, 诱导率100%; 继代最适培养基为MS+TDZ 0.1 mg·L^-1+蔗糖20 g·L^-1, 平均增殖系数5.64; 培养基中同时添加2.5 mg·L^-1 6-BA和1.5 mg·L^-1 IBA壮苗效果明显; 最佳生根培养基为1/3MS+NAA 2 mg·L^-1+IAA 10 mg·L^-1+抗坏血酸50 g·L^-1+柠檬酸50 g·L^-1+蔗糖10 g·L^-1, 生根率达80%以上。

关键词：武夷岩茶; 种胚; 组织培养; 快速繁殖

收稿：2016-11-18   修定：2017-03-20

资助：福建省产业技术联合创新项目(闽发改高技[2014]514号)和泉州科技计划项目(2016N0031和2015N47)。

Tissue culture and rapid propagation of Camellia sinensis Dahongpao

TIAN Ao-Lei¹, GAO Jun-Jie², LI Dan-Dan¹, LIU Jian-Fu^1,*, WANG Ming-Yuan¹, XU Mao-Xing³, ZHANG Bin⁴

¹Department of Horticulture, Huaqiao University, Xiamen, Fujian 361021, China; ²Quanzhou Municipal Bureau of Agriculture Planting Station, Quanzhou, Fujian 362000, China; ³Wuyishan Mnicipal Bureau of Tea Industry, Nanping, Fujian 354300, China; ⁴Tea Factory of Xianmingyan in Wuyishan County, Nanping, Fujian 354300, China

Corresponding author: LIU Jian-Fu; E-mail: jianfu@hqu.edu.cn

Abstract:

The aseptic embryos of Camellia sinensis Dahongpao were used as explants, and the whole tissue culture system was established through the selection of embryo induction, subculture and root induction culture. The results showed that the optimum induction medium was MS+sucrose 30 g·L^-1 and the induction rate was 100%. The optimal medium for subculture was MS+TDZ 0.1 mg·L^-1+sucrose 20 g·L^-1, the average increment coefficient was 5.64. The effect of 2.5 mg·L^-1 6-BA and 1.5 mg·L^-1 IBA on seedling growth was obvious. The optimal rooting medium was 1/3MS+NAA 2.0 mg·L^-1+IAA 10.0 mg·L^-1+ascorbic acid 50 g·L^-1+citric acid 50 g·L^-1+sucrose 10 g·L^-1+agar 5.5 g·L^-1, the rooting rate was more than 80%.

Key words: Wuyi rock tea (Camellia sinensis Dahongpao); seed embryo; tissue culture; rapid propagation